|Title:||Single-cell RNA-seq reveals changes in cell cycle and differentiation programs upon aging of hematopoietic stem cells|
|Date:||April 12, 2016 (Tuesday)|
|Time:||12:00 noon - 1:00 p.m.|
|Venue:||Room 121, 1/F, Ho Sin-hang Engineering Building,
The Chinese University of Hong Kong,
|Speaker:||Professor Tin-lap Lee
School of Biomedical Sciences
Both intrinsic cell state changes and variations in the composition of stem cell populations have been implicated as contributors to aging. We used single-cell RNA-seq to dissect variability in hematopoietic stem cell (HSC) and hematopoietic progenitor cell populations from young and old mice from two strains. We found that cell cycle dominates the variability within each population and that there is a lower frequency of cells in the G1 phase among old compared with young long-term HSCs, suggesting that they traverse through G1 faster. Moreover, transcriptional changes in HSCs during aging are inversely related to those upon HSC differentiation, such that old short-term (ST) HSCs resemble young long-term (LT-HSCs), suggesting that they exist in a less differentiated state. Our results indicate both compositional changes and intrinsic, population-wide changes with age and are consistent with a model where a relationship between cell cycle progression and self-renewal versus differentiation of HSCs is affected by aging and may contribute to the functional decline of old HSCs.
Dr. Tin-lap Lee is an Associate Professor in the School of Biomedical Sciences. He is interested in genomic regulations of non-coding RNAs in stem cell development, germline stem cell biology, nanotoxicology and nanomedicine, and system biology modeling and algorithm development.
Enquiries: Miss Ricola Lo at tel 3943 8440
For more information, please refer to http://www.cse.cuhk.edu.hk/seminar.